Gene Expression of Antioxidant Enzymes in Healthy and Aborted Women

Gene expression analysis is one of the most important tools for discovering the link between human disorders and the genes that control them. The primary purpose of this study is to identify the gene expression profile of antioxidant enzymes in healthy and aborted mothers. The study comprised fifty-five mothers who were admitted to Al-Khansa'a Hospital in Mosul City. Ten mothers were healthy and had normal births, while the remaining forty-five mothers were divided into four groups: women who had abortions, mothers who had normal deliveries but prior miscarriages, aborted women with hypertension, and aborted women without hypertension. Gene analysis of placentas and aborted foetuses was performed using quantitative real-time PCR (q-PCR). In contrast to other mothers without hypertension and normal labour who had previously miscarried, as well as healthy mothers who had normal labour, the results showed significant variations in the gene expression profile of antioxidant enzymes, indicating highly down regulation of Glutathione Peroxidase, Superoxide Dismutase, and Catalase genes in the placenta tissues of aborted mothers with hypertension. According to their regulatory genes, mothers who have had abortions have higher oxidative stress due to downregulation of antioxidant gene expression. The placentas of hypertensive moms who had abortions showed reduced Glutathione Peroxidase, Superoxide Dismutase, and Catalase capabilities. Due to our outcomes, oxidative stress intensifies due to lower antioxidant enzyme activity in aborted moms with and without hypertension. Antioxidant enzymes are required during pregnancy to protect mothers from abortion, and they have been linked to miscarriages.