Transformation of intact yeast cells treated with alkali cations

Hisao Ito; Y Fukuda; Kousaku Murata; Akira Kimura · Journal of Bacteriology · 1983

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Author / responsible party

Hisao Ito; Y Fukuda; Kousaku Murata; Akira Kimura

Publisher

Journal of Bacteriology

Year

1983

Language

English

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Intact yeast cells treated with alkali cations took up plasmid DNA. Li+, Cs+, Rb+, K+, and Na+ were effective in inducing competence. Conditions for the transformation of Saccharomyces cerevisiae D13-1A with plasmid YRp7 were studied in detail with CsCl. The optimum incubation time was 1 h, and the optimum cell concentration was 5 x 10(7) cells per ml. The optimum concentration of Cs+ was 1.0 M. Transformation efficiency increased with increasing concentrations of plasmid DNA. Polyethylene glycol was absolutely required. Heat pulse and various polyamines or basic proteins stimulated the uptake of plasmid DNA. Besides circular DNA, linear plasmid DNA was also taken up by Cs+-treated yeast cells, although the uptake efficiency was considerably reduced. The transformation efficiency with Cs+ or Li+ was comparable with that of conventional protoplast methods for a plasmid containing ars1, although not for plasmids containing a 2 microns origin replication.

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APA 7

Ito, H, Fukuda, Y, Murata, K, & Kimura, A. (1983). Transformation of intact yeast cells treated with alkali cations. https://doi.org/10.1128/jb.153.1.163-168.1983

MLA

Ito, Hisao, et al. "Transformation of intact yeast cells treated with alkali cations." 1983. https://doi.org/10.1128/jb.153.1.163-168.1983.

Chicago

Ito, Hisao, Y Fukuda, Kousaku Murata, and Akira Kimura. 1983. "Transformation of intact yeast cells treated with alkali cations.". https://doi.org/10.1128/jb.153.1.163-168.1983.

Harvard

Ito, H. et al. 1983, Transformation of intact yeast cells treated with alkali cations, Journal of Bacteriology, available at: https://doi.org/10.1128/jb.153.1.163-168.1983 [Accessed 9 Aug. 2026].

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Title: Transformation of intact yeast cells treated with alkali cations

Author / contributors: Hisao Ito; Y Fukuda; Kousaku Murata; Akira Kimura

Publisher: Journal of Bacteriology

Publication year: 1983

Language: English

Subjects

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Plasmid; Transformation (genetics); Protoplast; Saccharomyces cerevisiae; Transformation efficiency; Biology; DNA; Plasmid preparation; Yeast; Polyethylene glycol; DNA replication; Incubation