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Hemolytic activity of alternative complement pathway as an indicator of innate immunity in pacu (Piaractus mesopotamicus)

Jaqueline Dalbello Biller-Takahashi et al · Sociedade Brasileira de Zootecnia · 2012

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Author / responsible party
Jaqueline Dalbello Biller-Takahashi et al
Publisher
Sociedade Brasileira de Zootecnia
Year
2012
ISSN
1516-3598
ISSN
1516-3598
Language
English
Serial publication

Acasalamento dirigido para aumentar a produção de animais geneticamente superiores e reduzir a variabilidade da progênie em bovinos Mating strategy for increasing the probability of producing outstanding animals and reducing progeny variability

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The objective of this study was to evaluate the methodology to establish the hemolytic activity of alternative complement pathway as an indicator of the innate immunity in Brazilian fish pacu (Piaractus mesopotamicus), in addition to verifying the influence of β-glucan as an immunostimulant. Fish were fed with diets containing 0, 0.1 and 1% β-glucan, during seven days, and then inoculated with Aeromonas hydrophila. Seven days after the challenge, they were bled for serum extraction. The methodology consisted of a kinetic assay that allows calculating the required time for serum proteins of the complement to promote 50% lysis of a rabbit red blood cell suspension. The method developed in mammals was successfully applied for pacu and determined that the hemolytic activity of the proteins of the complement system (alternative pathway) increased after the pathogen challenge, but was not influenced by the β-glucan treatment.

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APA 7

al, J. D. B. T. E. (2012). Hemolytic activity of alternative complement pathway as an indicator of innate immunity in pacu (Piaractus mesopotamicus). https://doi.org/10.1590/S1516-35982012000200001

MLA

al, Jaqueline Dalbello Biller-Takahashi et. "Hemolytic activity of alternative complement pathway as an indicator of innate immunity in pacu (Piaractus mesopotamicus)." 2012. https://doi.org/10.1590/S1516-35982012000200001.

Chicago

al, Jaqueline Dalbello Biller-Takahashi et. 2012. "Hemolytic activity of alternative complement pathway as an indicator of innate immunity in pacu (Piaractus mesopotamicus).". https://doi.org/10.1590/S1516-35982012000200001.

Harvard

al, J. D. B. T. E. 2012, Hemolytic activity of alternative complement pathway as an indicator of innate immunity in pacu (Piaractus mesopotamicus), Sociedade Brasileira de Zootecnia, available at: https://doi.org/10.1590/S1516-35982012000200001 [Accessed 10 Aug. 2026].

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Title
Hemolytic activity of alternative complement pathway as an indicator of innate immunity in pacu (Piaractus mesopotamicus)
Author / contributors
Jaqueline Dalbello Biller-Takahashi et al
Publisher
Sociedade Brasileira de Zootecnia
Publication year
2012
ISSN
1516-3598
ISSN
1516-3598
Language
English

Subjects

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A. hydrophila; β-glucan; immune response; pacu
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