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Cryotop and development of vitrified immature bovine oocytes

H Hajarian et al · Universidade Federal de Minas Gerais, Escola de Veterinária · 2011

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Author / responsible party
H Hajarian et al
Publisher
Universidade Federal de Minas Gerais, Escola de Veterinária
Year
2011
ISSN
1678-4162
ISSN
1678-4162
Language
English
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The effectiveness of different cryodevices (open-pulled straw (OPS), electron microscopy grid (EMG), and Cryotop was evaluated for vitrification of immature bovine oocytes. Polar body, metaphase II stage (MII), survivability, and subsequent developmental rates were determined. Only oocytes with four or five layers of cumulus cells were used. Oocytes were equilibrated in two vitrification solutions - 1: 10% DMSO + 10% ethylene glycol (EG) for 30-45sec and 2: 20% DMSO + 20% EG +0.5M sucrose for 25sec -, mounted on one of the cryodevices and directly plunged into liquid nitrogen for 10 days. Immature vitrified oocytes using Cryotop showed the highest rates of polar body extrusion (PB) and nuclear maturity (MII); 41 and 58% respectively. Vitrified oocytes using OPS and EMG showed 26 and 32%; and 35 and 46% of PB and MII rates, respectively. The highest survivability resulted from Cryotop and EMG groups and no significant difference was found between them. Vitrified oocytes using Cryotop had the highest cleavage and blastocyst rates. All of the mean rates for vitrified immature oocytes were significantly lower than that of control group (P<0.05). The results of this study showed the superiority of Cryotop device for vitrification of immature bovine oocytes

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APA 7

al, H. H. E. (2011). Cryotop and development of vitrified immature bovine oocytes. https://doi.org/10.1590/S0102-09352011000100011

MLA

al, H Hajarian et. "Cryotop and development of vitrified immature bovine oocytes." 2011. https://doi.org/10.1590/S0102-09352011000100011.

Chicago

al, H Hajarian et. 2011. "Cryotop and development of vitrified immature bovine oocytes.". https://doi.org/10.1590/S0102-09352011000100011.

Harvard

al, H. H. E. 2011, Cryotop and development of vitrified immature bovine oocytes, Universidade Federal de Minas Gerais, Escola de Veterinária, available at: https://doi.org/10.1590/S0102-09352011000100011 [Accessed 7 Aug. 2026].

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Title
Cryotop and development of vitrified immature bovine oocytes
Author / contributors
H Hajarian et al
Publisher
Universidade Federal de Minas Gerais, Escola de Veterinária
Publication year
2011
ISSN
1678-4162
ISSN
1678-4162
Language
English

Subjects

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bovino; oócito imaturo; vitrificação; dispositivos de congelamento
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