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Simultaneous Determination of Three Bioactive Compounds in Traditional Chinese Medicine Patent Prescription Zuojin Pill by HPLC

Cai, Jinzhang et al · SEDICI UNLP · 2012

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Author / responsible party
Cai, Jinzhang et al
Publisher
SEDICI UNLP
Year
2012
Language
English

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A high-performance liquid chromatography (HPLC) method coupled with ultraviolet (UV) detection has been developed and validated for simultaneous quantification of berberine hydrochloride, evodiamine and rutaecarpine in Zuojin Pill. Chromatographic separation were achieved on a ODS-C18 column (250×4.6 mm i.d., 5.0 μm particle) through a 25 min gradient delivery of a mixture of A (acetonitrile) and B (0.3 % phosphoric acid-0.3 % triethylamine) at a flow rate of 0.8 mL/min at 40 °C. All of the analytes showed good linearity (r > 0.999). The method was validated for repeatability, precision, stability, accuracy and selectivity. The validated method was applied to quality control of Zuojin Pill from different manufacture. Colegio de Farmacéuticos de la Provincia de Buenos Aires

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APA 7

Cai, J. E. A. (2012). Simultaneous Determination of Three Bioactive Compounds in Traditional Chinese Medicine Patent Prescription Zuojin Pill by HPLC. http://sedici.unlp.edu.ar/handle/10915/18253

MLA

Cai, Jinzhang et al. "Simultaneous Determination of Three Bioactive Compounds in Traditional Chinese Medicine Patent Prescription Zuojin Pill by HPLC." 2012. http://sedici.unlp.edu.ar/handle/10915/18253.

Chicago

Cai, Jinzhang et al. 2012. "Simultaneous Determination of Three Bioactive Compounds in Traditional Chinese Medicine Patent Prescription Zuojin Pill by HPLC.". http://sedici.unlp.edu.ar/handle/10915/18253.

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Cai, J. E. A. 2012, Simultaneous Determination of Three Bioactive Compounds in Traditional Chinese Medicine Patent Prescription Zuojin Pill by HPLC, SEDICI UNLP, available at: http://sedici.unlp.edu.ar/handle/10915/18253 [Accessed 9 Aug. 2026].

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Title
Simultaneous Determination of Three Bioactive Compounds in Traditional Chinese Medicine Patent Prescription Zuojin Pill by HPLC
Author / contributors
Cai, Jinzhang et al
Publisher
SEDICI UNLP
Publication year
2012
Language
English

Subjects

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Farmacia; Farmacología; berberine hydrochloride; evodiamine; HPLC; rutaecarpine; Zuojin pill; Medicina Tradicional
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