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Localization STED (LocSTED) microscopy with 15 nm resolution

Puthukodan Sujitha et al · Wiley · 2020

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Author / responsible party
Puthukodan Sujitha et al
Publisher
Wiley
Year
2020
ISSN
2192-8614
ISSN
2192-8614
Language
English
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We present localization with stimulated emission depletion (LocSTED) microscopy, a combination of STED and single-molecule localization microscopy (SMLM). We use the simplest form of a STED microscope that is cost effective and synchronization free, comprising continuous wave (CW) lasers for both excitation and depletion. By utilizing the reversible blinking of fluorophores, single molecules of Alexa 555 are localized down to ~5 nm. Imaging fluorescently labeled proteins attached to nanoanchors structured by STED lithography shows that LocSTED microscopy can resolve molecules with a resolution of at least 15 nm, substantially improving the classical resolution of a CW STED microscope of about 60 nm. LocSTED microscopy also allows estimating the total number of proteins attached on a single nanoanchor.

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APA 7

al, P. S. E. (2020). Localization STED (LocSTED) microscopy with 15 nm resolution. https://doi.org/10.1515/nanoph-2019-0398

MLA

al, Puthukodan Sujitha et. "Localization STED (LocSTED) microscopy with 15 nm resolution." 2020. https://doi.org/10.1515/nanoph-2019-0398.

Chicago

al, Puthukodan Sujitha et. 2020. "Localization STED (LocSTED) microscopy with 15 nm resolution.". https://doi.org/10.1515/nanoph-2019-0398.

Harvard

al, P. S. E. 2020, Localization STED (LocSTED) microscopy with 15 nm resolution, Wiley, available at: https://doi.org/10.1515/nanoph-2019-0398 [Accessed 7 Aug. 2026].

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Title
Localization STED (LocSTED) microscopy with 15 nm resolution
Author / contributors
Puthukodan Sujitha et al
Publisher
Wiley
Publication year
2020
ISSN
2192-8614
ISSN
2192-8614
Language
English

Subjects

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stimulated emission depletion (sted) microscopy; single-molecule localization microscopy (smlm); protein nanoanchors; single-molecule imaging
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