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Spectrophotometric Determination of Chloramphenicol in Pharmaceutical Preparations

Tamathir A. Hamoudi et al · University of Mosul, College of Education for Pure Science · 2018

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Author / responsible party
Tamathir A. Hamoudi et al
Publisher
University of Mosul, College of Education for Pure Science
Year
2018
ISSN
1812-125X
ISSN
1812-125X
Language
English
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A spectrophotometric method for the determination of trace amounts of chloramphenicol has been proposed. This method depends upon the reduction of the nitro to amino group, condensation with promethazine reagent in the presence of cerium (IV) ions to form a colored dye which exhibits maximum absorption at 606 nm with a high sensitivity (molar absorptivity is 1.292×104 l.mol-1.cm-1, and Sandell's sensitivity index of 0.025 μg/cm2). Beer' s law is applied within the concentration range of obeyed in 0.4 to 12 ppm with a relative error of -2.73 to + 0.37% and a relative standard deviation of ± 3.84 to ± 0.44%, depending on the concentration level. The method has been successfully applied to the determination of chloramphenicol in pharmaceutical preparations (capsules, eye drop and eye ointment).

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APA 7

al, T. A. H. E. (2018). Spectrophotometric Determination of Chloramphenicol in Pharmaceutical Preparations. https://doi.org/10.33899/edusj.2018.159308

MLA

al, Tamathir A. Hamoudi et. "Spectrophotometric Determination of Chloramphenicol in Pharmaceutical Preparations." 2018. https://doi.org/10.33899/edusj.2018.159308.

Chicago

al, Tamathir A. Hamoudi et. 2018. "Spectrophotometric Determination of Chloramphenicol in Pharmaceutical Preparations.". https://doi.org/10.33899/edusj.2018.159308.

Harvard

al, T. A. H. E. 2018, Spectrophotometric Determination of Chloramphenicol in Pharmaceutical Preparations, University of Mosul, College of Education for Pure Science, available at: https://doi.org/10.33899/edusj.2018.159308 [Accessed 8 Aug. 2026].

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Title
Spectrophotometric Determination of Chloramphenicol in Pharmaceutical Preparations
Author / contributors
Tamathir A. Hamoudi et al
Publisher
University of Mosul, College of Education for Pure Science
Publication year
2018
ISSN
1812-125X
ISSN
1812-125X
Language
English

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chloramphenicol; pharmaceutical preparations; cerium
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