Effect of alternative carbohydrates on micropropagation in plant species with special reference to Vanilla planifolia Jacks

One of the challenges posed by cultivating certain agricultural species, such as Vanilla planifolia Jacks., is obtaining high-quality plantlets to meet the high global demand for vanillin. However, despite the progress achieved, this crop is severely damaged by fungal infections such as Fusarium oxysporum f. sp. vanillae (Fov). Through in vitro selection, the RH350 genotype, which shows a certain level of resistance to this pathogen, is currently available. Therefore, in this study, it was proposed to review the role of different concentrations and sources of carbohydrates in the micropropagation of various plant species. According to the literature, sucrose is the most commonly used carbohydrate, probably because it contributes to greater shoot proliferation and shoot growth. Considering that vegetative propagation techniques require a precise propagation protocol to obtain plants faithful to the promising genotype of interest, it was proposed to develop the present literature review to evaluate the advances obtained in this area between 2000 and 2025. The impact of different carbon concentration and sources is the primary focus of the micropropagation process of the valuable genotype RH350 which was obtained through in vitro selection, against Fov filtrates for future field evaluation by vanilla producers.