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Multiplexed stimulated emission depletion nanoscopy (mSTED) for 5-color live-cell long-term imaging of organelle interactome

Yuran Huang et al · Editorial Office of Opto-Electronic Journals Group, Institute of Optics and Electronics, CAS, China · 2024

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Author / responsible party
Yuran Huang et al
Publisher
Editorial Office of Opto-Electronic Journals Group, Institute of Optics and Electronics, CAS, China
Year
2024
ISSN
2096-4579
ISSN
2096-4579
Language
English

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Stimulated emission depletion microscopy (STED) holds great potential in biological science applications, especially in studying nanoscale subcellular structures. However, multi-color STED imaging in live-cell remains challenging due to the limited excitation wavelengths and large amount of laser radiation. Here, we develop a multiplexed live-cell STED method to observe more structures simultaneously with limited photo-bleaching and photo-cytotoxicity. By separating live-cell fluorescent probes with similar spectral properties using phasor analysis, our method enables five-color live-cell STED imaging and reveals long-term interactions between different subcellular structures. The results here provide an avenue for understanding the complex and delicate interactome of subcellular structures in live-cell.

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APA 7

al, Y. H. E. (2024). Multiplexed stimulated emission depletion nanoscopy (mSTED) for 5-color live-cell long-term imaging of organelle interactome. https://doi.org/10.29026/oea.2024.240035

MLA

al, Yuran Huang et. "Multiplexed stimulated emission depletion nanoscopy (mSTED) for 5-color live-cell long-term imaging of organelle interactome." 2024. https://doi.org/10.29026/oea.2024.240035.

Chicago

al, Yuran Huang et. 2024. "Multiplexed stimulated emission depletion nanoscopy (mSTED) for 5-color live-cell long-term imaging of organelle interactome.". https://doi.org/10.29026/oea.2024.240035.

Harvard

al, Y. H. E. 2024, Multiplexed stimulated emission depletion nanoscopy (mSTED) for 5-color live-cell long-term imaging of organelle interactome, Editorial Office of Opto-Electronic Journals Group, Institute of Optics and Electronics, CAS, China, available at: https://doi.org/10.29026/oea.2024.240035 [Accessed 8 Aug. 2026].

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Title
Multiplexed stimulated emission depletion nanoscopy (mSTED) for 5-color live-cell long-term imaging of organelle interactome
Author / contributors
Yuran Huang et al
Publisher
Editorial Office of Opto-Electronic Journals Group, Institute of Optics and Electronics, CAS, China
Publication year
2024
ISSN
2096-4579
ISSN
2096-4579
Language
English

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optical nanoscopy; phasor analysis; multicolor live cell imaging
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