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Genetic engineering and single vector expression of VP1 and VP2 proteins of chicken anemia virus in different lineages of Escherichia coli

C.F. Prezotto et al · Universidade Federal de Minas Gerais, Escola de Veterinária · 2026

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Author / responsible party
C.F. Prezotto et al
Publisher
Universidade Federal de Minas Gerais, Escola de Veterinária
Year
2026
ISSN
1678-4162
ISSN
1678-4162
Language
English
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ABSTRACT The validity of genetic engineering of virus genes as an essential tool for the development of more effective and safe vaccines has been proven recently with SARS CoV-2 mRNA and vectored vaccines. The epidemiological risk of employing the chicken anemia virus (CAV) active virus vaccines may be circumvented using such type of vaccine strategies. CAV genome expresses three proteins, including the single structural capsid protein VP1. VP1 is dependent on VP2 for virion assembly and to adequately expressing its own neutralizing antibodies inducing epitopes. This study describes cloning of the VP1 and VP2 genes in the same plasmid (pET100) and analyzing their expression in different strains of E. coli.

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APA 7

al, C. P. E. (2026). Genetic engineering and single vector expression of VP1 and VP2 proteins of chicken anemia virus in different lineages of Escherichia coli. https://doi.org/10.1590/1678-4162-13476

MLA

al, C.F. Prezotto et. "Genetic engineering and single vector expression of VP1 and VP2 proteins of chicken anemia virus in different lineages of Escherichia coli." 2026. https://doi.org/10.1590/1678-4162-13476.

Chicago

al, C.F. Prezotto et. 2026. "Genetic engineering and single vector expression of VP1 and VP2 proteins of chicken anemia virus in different lineages of Escherichia coli.". https://doi.org/10.1590/1678-4162-13476.

Harvard

al, C. P. E. 2026, Genetic engineering and single vector expression of VP1 and VP2 proteins of chicken anemia virus in different lineages of Escherichia coli, Universidade Federal de Minas Gerais, Escola de Veterinária, available at: https://doi.org/10.1590/1678-4162-13476 [Accessed 7 Aug. 2026].

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Title
Genetic engineering and single vector expression of VP1 and VP2 proteins of chicken anemia virus in different lineages of Escherichia coli
Author / contributors
C.F. Prezotto et al
Publisher
Universidade Federal de Minas Gerais, Escola de Veterinária
Publication year
2026
ISSN
1678-4162
ISSN
1678-4162
Language
English

Subjects

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Gyrovirus chickenanemia; Anelloviridae; Gallus gallus domesticus; gene expression; three-dimensional epitopes
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