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Recombinant gp19 as a potential antigen for detecting anti-Ehrlichia canis antibodies in dog sera

Rômulo Silva de Oliveira et al · Colégio Brasileiro de Parasitologia Veterinária

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Author / responsible party
Rômulo Silva de Oliveira et al
Publisher
Colégio Brasileiro de Parasitologia Veterinária
ISSN
1984-2961
ISSN
1984-2961
Language
English

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The canine monocytic ehrlichiosis, caused by Ehrlichia canis, is endemic in several regions of Brazil. Some serological diagnostic techniques using immunodominant proteins of E. canis as antigens are available, but their specificities and sensitivities are questionable. Based on this, the objective of this study was to test the antigenic potential of the recombinant gp19 protein (rGP19) for subsequent use in diagnostic tests. The rGP19 expressed in the Escherichia coli strain BL21 (DE3) C41 was recognized in the sera from experimentally infected dogs using ELISA and Western blotting. Thus, it was possible to obtain a promising antigen with the ability to differentiate between E. canis-positive and -negative animals, even 1 week after infection.

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APA 7

al, R. S. D. O. E. (s. f.). Recombinant gp19 as a potential antigen for detecting anti-Ehrlichia canis antibodies in dog sera. https://doi.org/10.1590/S1984-29612015055

MLA

al, Rômulo Silva de Oliveira et. "Recombinant gp19 as a potential antigen for detecting anti-Ehrlichia canis antibodies in dog sera.". https://doi.org/10.1590/S1984-29612015055.

Chicago

al, Rômulo Silva de Oliveira et. s. f. "Recombinant gp19 as a potential antigen for detecting anti-Ehrlichia canis antibodies in dog sera.". https://doi.org/10.1590/S1984-29612015055.

Harvard

al, R. S. D. O. E. s. f, Recombinant gp19 as a potential antigen for detecting anti-Ehrlichia canis antibodies in dog sera, Colégio Brasileiro de Parasitologia Veterinária, available at: https://doi.org/10.1590/S1984-29612015055 [Accessed 10 Aug. 2026].

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Title
Recombinant gp19 as a potential antigen for detecting anti-Ehrlichia canis antibodies in dog sera
Author / contributors
Rômulo Silva de Oliveira et al
Publisher
Colégio Brasileiro de Parasitologia Veterinária
ISSN
1984-2961
ISSN
1984-2961
Language
English

Subjects

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Diagnosis; serology; rickettsiae; ehrlichiosis
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