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Mesoporous titania thin films as efficient enzyme carriers for paraoxon determination/detoxification: effects of enzyme binding and pore hierarchy in biocatalyst activity and reusability

Francica, Cynthia et al · Royal Society of Chemistry · 2014

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Author / responsible party
Francica, Cynthia et al
Publisher
Royal Society of Chemistry
Year
2014
ISSN
3127-3136
ISSN
3127-3136
Language
English

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The aims of this work were to efficiently immobilize organophosphate degrading enzyme, His6-OPH, on hierarchical mesoporoustitania thin films and further, to use this biocatalyst films as efficient tools in the detection/detoxofication of pesticides, paraoxon in our case. His6-OPH was immobilized on mesoporous thin films with uniform (9nm) and bimodal (13-38nm) pore size distribution, through covalent attachment and physical adsorption. The biocatalyst films show good activity, and enhanced stability with respect to the free enzyme at extreme conditions of pH and temperature, especially around neutral pH and room temperature. In addition, they can be easily separated from the reaction media and reused multiple times without significant loss in activity. Fil: Francica, Cynthia. University of Maribor. Faculty of Mechanical Engineering. Centre of Sensor Technology; Eslovenia Fil: Bellino, Martin Gonzalo. Comisión Nacional de Energía Atómica. Gerencia del Área de Seguridad Nuclear y Ambiente. Gerencia de Química (CAC); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

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APA 7

Francica, C. E. A. (2014). Mesoporous titania thin films as efficient enzyme carriers for paraoxon determination/detoxification: effects of enzyme binding and pore hierarchy in biocatalyst activity and reusability. http://hdl.handle.net/11336/32856

MLA

Francica, Cynthia et al. "Mesoporous titania thin films as efficient enzyme carriers for paraoxon determination/detoxification: effects of enzyme binding and pore hierarchy in biocatalyst activity and reusability." 2014. http://hdl.handle.net/11336/32856.

Chicago

Francica, Cynthia et al. 2014. "Mesoporous titania thin films as efficient enzyme carriers for paraoxon determination/detoxification: effects of enzyme binding and pore hierarchy in biocatalyst activity and reusability.". http://hdl.handle.net/11336/32856.

Harvard

Francica, C. E. A. 2014, Mesoporous titania thin films as efficient enzyme carriers for paraoxon determination/detoxification: effects of enzyme binding and pore hierarchy in biocatalyst activity and reusability, Royal Society of Chemistry, available at: http://hdl.handle.net/11336/32856 [Accessed 7 Aug. 2026].

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Title
Mesoporous titania thin films as efficient enzyme carriers for paraoxon determination/detoxification: effects of enzyme binding and pore hierarchy in biocatalyst activity and reusability
Author / contributors
Francica, Cynthia et al
Publisher
Royal Society of Chemistry
Publication year
2014
ISSN
3127-3136
ISSN
3127-3136
Language
English

Subjects

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Hierarchical Mesoporous Thin Films; Tio2; Adsorption/Covalent Attachment; Organophosphate Hydrolysis; https://purl.org/becyt/ford/2.10; https://purl.org/becyt/ford/2
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