News Help Institutional access
Back to results
Bibliographic record · Consultation and access
Libro

In vivo Protein Crosslinking and Co-Immunoprecipitation in Haloferax volcanii

Paggi, Roberto Alejandro et al · Springer · 2022

Open-access full text
Quick overview. Review the resource’s basic details, then access the content using the main button. This page shows only the information needed to identify, cite, and open the work.
Author / responsible party
Paggi, Roberto Alejandro et al
Publisher
Springer
Year
2022
ISSN
0716-2445
ISSN
0716-2445
Language
English

Resource access

Open the content from the main option or choose another available source.

CONICET Digital CONICET Digital OAI-PMH
Entrar por CONICET Digital
Main access

Open-access full text

Texto completo identificado como acceso abierto.
Open text

Summary

Descripción general del contenido del recurso.

Co-Immunoprecitation is a powerful and commonly used method to identify protein-protein interactions in a physiological context. Here, we report a Co-Immunoprecitation Co-IP protocol that was adapted and optimized for the haloarchaeon Haloferax volcanii to identify interacting partners to the LonB protease. This protocol includes the in vivo crosslinking of H. volcanii proteins using two different crosslinker agents, dithiobis(succinimidyl propionate) and formaldehyde, followed by immunoprecipitation with anti-LonB antibody conjugated to Protein A−Sepharose beads. Tryptic on-bead protein digestion was performed combined with Mass Spectrometry analysis of peptides for the identification and quantification of LonB ligands. Fil: Paggi, Roberto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina Fil: de Castro, Rosana Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina

How to cite

Elegí el formato que necesitás y copiá la referencia al portapapeles.

APA 7

Paggi, R. A. E. A. (2022). In vivo Protein Crosslinking and Co-Immunoprecipitation in Haloferax volcanii. Springer. http://hdl.handle.net/11336/228867

MLA

Paggi, Roberto Alejandro et al. In vivo Protein Crosslinking and Co-Immunoprecipitation in Haloferax volcanii. Springer, 2022. http://hdl.handle.net/11336/228867.

Chicago

Paggi, Roberto Alejandro et al. 2022. In vivo Protein Crosslinking and Co-Immunoprecipitation in Haloferax volcanii. Springer. http://hdl.handle.net/11336/228867.

Harvard

Paggi, R. A. E. A. 2022, In vivo Protein Crosslinking and Co-Immunoprecipitation in Haloferax volcanii, Springer, available at: http://hdl.handle.net/11336/228867 [Accessed 7 Aug. 2026].

Share and print

Save the record, copy its permanent link, or print it as a PDF.

Export reference

You can export the record in common formats for use in a reference manager.

RIS BibTeX

Resource details

Bibliographic information to help confirm that this is the correct material.

Title
In vivo Protein Crosslinking and Co-Immunoprecipitation in Haloferax volcanii
Author / contributors
Paggi, Roberto Alejandro et al
Publisher
Springer
Publication year
2022
ISSN
0716-2445
ISSN
0716-2445
Language
English

Subjects

Explore related resources through these subjects.

IN VIVO CROSS-LINKING; CO-IMMUNOPRECIPITATION; HALOARCHAEA; HALOFERAX VOLCANII; PROTEIN-PROTEIN INTERACTION; https://purl.org/becyt/ford/1.6; https://purl.org/becyt/ford/1
Copied