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Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

Don W. Cleveland; S G Fischer; Marc W. Kirschner; Ulrich K. Laemmli · Journal of Biological Chemistry · 1977

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Author / responsible party
Don W. Cleveland; S G Fischer; Marc W. Kirschner; Ulrich K. Laemmli
Publisher
Journal of Biological Chemistry
Year
1977
Language
English

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Summary

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A rapid and convenient method for peptide mapping of proteins has been developed. The technique, which is especially suitable for analysis of proteins that have been isolated from gels containg sodium dodecyl sulfate, involves partial enzymatic proteolysis in the presence of sodium dodecyl sulfate and analysis of the cleavage products by polyacrylamide gel electrophoresis. The pattern of peptide fragments produced is characteristic of the protein substrate and the proteolytic enzyme and is highly reproducible. Several common proteases have been used including chymotrypsin, Staphylococcus aureus protease, and papain.

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APA 7

Cleveland, D. W, Fischer, S. G, Kirschner, M. W, & Laemmli, U. K. (1977). Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. https://doi.org/10.1016/s0021-9258(19)75212-0

MLA

Cleveland, Don W, et al. "Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis." 1977. https://doi.org/10.1016/s0021-9258(19)75212-0.

Chicago

Cleveland, Don W, S G Fischer, Marc W. Kirschner, and Ulrich K. Laemmli. 1977. "Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.". https://doi.org/10.1016/s0021-9258(19)75212-0.

Harvard

Cleveland, D. W. et al. 1977, Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis, Journal of Biological Chemistry, available at: https://doi.org/10.1016/s0021-9258(19)75212-0 [Accessed 10 Aug. 2026].

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Resource details

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Title
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
Author / contributors
Don W. Cleveland; S G Fischer; Marc W. Kirschner; Ulrich K. Laemmli
Publisher
Journal of Biological Chemistry
Publication year
1977
Language
English

Subjects

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Proteolysis; Sodium dodecyl sulfate; Papain; Chemistry; Gel electrophoresis; Proteases; Protease; Peptide; Biochemistry; Chymotrypsin; Polyacrylamide gel electrophoresis; Chromatography
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