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Development and optimization of a high-performance liquid chromatography method for the determination of theaflavin purity

TIAN Hongyun et al · The Editorial Office of Chinese Journal of Food Hygiene · 2025

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Author / responsible party
TIAN Hongyun et al
Publisher
The Editorial Office of Chinese Journal of Food Hygiene
Year
2025
ISSN
1004-8456
ISSN
1004-8456
Language
zho
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ObjectivesA high-performance liquid chromatography method was developed to determine the purity of theaflavins， a food additive.MethodsThe samples were dissolved in ethanol-water （2∶8，V/V） solution and separated by C18 reversed-phase chromatography with acetonitrile and 0.1% phosphoric acid as mobile phase. The flow rate was 1 mL/min and the column temperature was 35 °C， the detection was performed at 278 nm using a UV detector.ResultsThe linear relationship of theaflavins was good in the range of 20-500 μg/mL， and the correlation coefficient r20.99， the limit of detection of the four components of tea polyphenols was 0.2 μg/mL， and the limit of quantification was 0.5 μg/mL.The recovery rate of the samples was 97.9%-101.3%， and the relative standard deviation was 0.3%-1.6%.ConclusionThe method has high precision， good stability， simple operation， and can meet the requirements for the purity determination of food additive theaflavin.

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APA 7

al, T. H. E. (2025). Development and optimization of a high-performance liquid chromatography method for the determination of theaflavin purity. https://doi.org/10.13590/j.cjfh.2025.03.005

MLA

al, TIAN Hongyun et. "Development and optimization of a high-performance liquid chromatography method for the determination of theaflavin purity." 2025. https://doi.org/10.13590/j.cjfh.2025.03.005.

Chicago

al, TIAN Hongyun et. 2025. "Development and optimization of a high-performance liquid chromatography method for the determination of theaflavin purity.". https://doi.org/10.13590/j.cjfh.2025.03.005.

Harvard

al, T. H. E. 2025, Development and optimization of a high-performance liquid chromatography method for the determination of theaflavin purity, The Editorial Office of Chinese Journal of Food Hygiene, available at: https://doi.org/10.13590/j.cjfh.2025.03.005 [Accessed 8 Aug. 2026].

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Title
Development and optimization of a high-performance liquid chromatography method for the determination of theaflavin purity
Author / contributors
TIAN Hongyun et al
Publisher
The Editorial Office of Chinese Journal of Food Hygiene
Publication year
2025
ISSN
1004-8456
ISSN
1004-8456
Language
zho

Subjects

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theaflavin; high-performance liquid chromatography; maximum absorption wavelength; accuracy; precision
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