Enrichment, separation, and content determination of five effective components in liquorice root tablets by IER-CNTs combined with DLSPE-HPLC

ObjectiveTo establish a double-layer solid phase extraction and high performance liquid chromatography （DLSPE-HPLC） method for the simultaneous determination of five active components， including Liquiritin， Isoglycyrrhizin， Glycyrrhizin， Glycyrrhizic Chalcone A and Glabridine， in medicinal and edible Chinese herbal Liquorice root tablets using macroporous weak base anion exchange resin-carbon nanotubes （IER-CNTs）. To provide reference for the quality and safety comprehensive evaluation of licorice decoction pieces.MethodsThe chromatography was performed on a Platisil ODS column with acetonitrile-0.05% phosphoric acid as mobile phase and gradient elution. The detection wavelength was set at 237 nm， the column temperature was 30 ℃， and the flow rate was 1.0 mL/min. The active components and their contents of liquorice samples were determined statistically and analyzed by component analysis.ResultsThe linear relationship of this method is good （r>0.999）， with a linear range of 0.5-100 μg/ml， a recovery rate of 94.58-97.45%， and a relative standard deviation of 1.3-3.2%. Five target components were detected in Liquorice root tablets， and the overall quality was good.ConclusionThis method is suitable for simultaneous determination and content analysis of 5 active components in Glycyrrhiza liquorice samples. Component analysis statistics can provide theoretical reference and methodological guidance for comprehensive evaluation of drug efficacy and quality safety of glycyrrhiza liquorrhiza in batch development.