Determination of 1,2-propanediol in milk using the solid phase extraction combined with gas chromatography-mass spectrometry

ObjectiveDeveloped a method based on solid phase extraction（SPE） combined with gas chromatography-mass spectrometry （GC-MS） to determine 1，2-propanediol in milk.MethodsThe precipitation technology of protein and solid phase extraction，pretreatment technology in milk testing，were carefully investigated. Protein of sample milk was precipitated by potassium ferrocyanide and zinc acetate， then cleaned-up by silicious earth SPE.ResultsIn the range of 0.1-50 μg/mL，1，2-propanediol had good linear with R2=0.999 4 under the selected conditions. The recovery and relative standard deviation were 81.8%-86.5 % and 3.1%-5.8% at 0.33， 0.5 and 2.0 mg/kg spiked. And the minimum detection concentration of the method was 0.1 mg/kg.ConclusionThe developed method has proved convenient， environmental- friendly， accurate and sensitive.